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US 4,374,928 AGranted
Novel reductase
AssigneeHoffmann-La Roche Inc.
InventorsNathan Brot, Herbert Weissbach
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Abstract
A novel reductase which is an enzyme that can catalyze the reduction of methionine sulfoxide resides in proteins is described. The enzyme has been found in E. coli, Euglena gracilis, Tetrahymena pyriformis, Hela cells, rat tissue and spinach. The enzyme has an estimated molecular weight of about 18,000-20,000. It can be used to restore biological activity to proteins inactivated by oxidation of methionine residues.
Claims7
Peptide MetSO reductase being an enzyme endogenous to biological cells and being essentially free of other endogenous peptides, said reductase being characterized as follows:
- (i) having the ability to reduce methionine sulfoxide residues in peptides to methionine;
- (ii) unable to reduce free methionine sulfoxide to methionine;
- (iii) having a molecular weight of about 18,000 to 20,000 (Ultragel);
- and
- (iv) being free of transacetylase activity.
2 more elements
Dependencies are inferred from the claim text.