E.Coli bacteria carrying recombinant plasmids and their use in the fermentative production of L-tryptophan
AssigneeAjinomoto Co., Inc.
InventorsDavid M. Anderson, Klaus M. Herrmann, Ronald L. Somerville
Life of this patent
Estimating the term…
Abstract
A bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase carrying a plasmid with genetic information to control L-tryptophan production is useful for the fermentative production of L-tryptophan in high yields.
Claims34
A bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase carrying a plasmid with genetic information to control tryptophan production, wherein said host is resistant to a tryptophan analog.
A bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase carrying a plasmid with genetic information to control tryptophan production, which host additionally carries a gene deficiency selected from the group consisting of trpR, aroP, pheA and combinations thereof.
A method of producing L-tryptophan by fermentation which comprises:
- growing a bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase, carrying a plasmid with genetic information to control tryptophan production in an appropriate growth medium, and collecting tryptophan from said medium;
- wherein said host is resistant to a tryptophan analog.
A method of producing L-tryptophan by fermentation which comprises:
- growing a bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase carrying a plasmid with genetic information to control tryptophan production in an appropriate growth medium, and collecting tryptophan from said medium;
- wherein said host additionally carries a gene deficiency selected from the group consisting of trpR, aroP, tyrA, pheA, and combinations thereof.
Dependencies are inferred from the claim text.