Fermentation methods of preparing ergostadientriols
AssigneeZ-L Limited Partnership
InventorFritz W. Zilliken
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Abstract
The present invention concerns ergostadientriols which may be recovered from either the fungus R. oligosporus or the fungus R. oryzae as well as from soybeans which have been fermented with either of these fungi. Alternatively, the sterols may be chemically synthesized. The sterols of this invention are antioxidants which may be used in anti-oxidant compositions alone or preferably with one or more isoflavone. Of particular significance is the discovery that the ergostadientriols of this invention are useful in lowering serum cholesterol levels. Accordingly, this invention in a particularly preferred embodiment discloses pharmaceutical compositions containing one or more ergostadientriol. Finally, therapeutic methods which utilize pharmaceutical compositions in accordance with this invention are disclosed.
Claims6
A method for preparing one or more compounds having the structure:
- ##STR13## wherein X may be hydrogen or a hydroxyl group provided that X twice be hydroxyl which comprises fermenting soybeans in presence of fungus R. oligosporus or R. oryzae, contacting the resulting fermented soybean product with methanol to separate from said fermented soybean product an extract containing said compound or compounds and isolating said compound or compounds from said methanol extract.
A method in accordance with claim 4 wherein the isolation of said compound or compounds from said methanol extract comprises chromatographic separation, said chromatographic separation being molecular sieve chromatography followed by adsorption chromatography.
Dependencies are inferred from the claim text.