Method and reagent for the determination of triglycerides
AssigneeBoehringer Mannheim GmbH
InventorsSigmar Klose, Albert Roder, Walter Schneider
Life of this patent
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Abstract
A method for the determination of triglycerides by ester cleavage utilizing lipase and optionally esterase with the formation of fatty acids and glycerol, phosphorylation of the glycerol with adnosine triphosphate in the presence of glycerol kinase with the formation of glycerol-1-phosphate and adenosine diphosphate and determination of one of the latter two products. The glycerol kinase used is from Bacillus stearothermophilis and acts in combination with at least one activator selected from the group consisting of detergents, phenol derivatives and aniline derivative.
Claims28
In a method for the determination of triglycerides which comprises combining sample, lipase, glycerol kinase, adenosine triphosphate, and at least one lipase activator selected from the group consisting of polyethylene oxide ester surfactants, polyethylene oxide ether surfactants, bile acid surfactants, alkyl sulphate surfactants, phenol derivatives, and analine derivatives, under conditions suitable to form glycerol-1-phosphate and adenosine diphosphate and determining the presence or concentration of either glycerol-1-phosphate or adenosine diphosphate, the improvement comprising using a glycerol kinase from Bacillus stereothermophilis.
Reagent for the determination of triglycerides comprising lipase, adenosine triphosphate, glycerokinase, a reagent system for determining glycerol-l-phosphate or adenosine diphosphate and a glycerokinase from Bacillus stearothermophilis, with at least one lipase activator selected from the group consisting of polyethylene oxide ester surfactants, polyethylene oxide ether surfactants, bile acid surfactants, alkyl sulphate surfactants, phenol derivatives and aniline derivatives.
Dependencies are inferred from the claim text.