Recombinant DNA transfer vectors

A recombinant plasmid vector comprising the nucleotide sequence coding for the growth hormone of an animal species and capable of transforming a microorganism, synthesized by a process comprising:

• isolating polyadenylated RNA from pituitary cells of the animal species,
• preparing double-stranded cDNA transcripts of the isolated RNA,
• fractionating the cDNA according to its molecular length, in order to produce a fraction enriched for cDNA coding for the growth hormone of the animal species,
• joining the cDNA coding for growth hormone covalently with a plasmid vector to produce a recombinant plasmid capable of transforming a microorganism.

A recombinant DNA transfer vector comprising codons for human growth hormone, comprising the nucleotide sequence:

• 5'-G GCL.sub.24 TTK.sub.25 GAK.sub.26 ACL.sub.27 TAK.sub.28 CAJ.sub.29 GAJ.sub.30 TTK.sub.31 GAJ.sub.32 GAJ.sub.33 ACL.sub.34 TAK.sub.35 ATM.sub.36 CCL.sub.37 AAJ.sub.38 GAJ.sub.39 CAJ.sub.40 AAJ.sub.41 TAK.sub.42 QR.sub.43 S.sub.43 TTK.sub.44 X.sub.45 TY.sub.45 CAJ.sub.46 AAK.sub.47 CCL.sub.48 CAJ.sub.49 ACL.sub.50 QR.sub.51 S.sub.51 X.sub.52 TY.sub.52 TGK.sub.53 TTK.sub.54 QR.sub.55 S.sub.55 GAJ.sub.56 QR.sub.57 S.sub.57 ATM.sub.58 CCL.sub.59 ACL.sub.60 CCL.sub.61 QR.sub.62 S.sub.62 AAK.sub.63 W.sub.64 GZ.sub.64 GAJ.sub.65 GAJ.sub.66 ACL.sub.67 CAJ.sub.68 CAJ.sub.69 AAJ.sub.70 QR.sub.71 S.sub.71 AAK.sub.72 X.sub.73 TY.sub.73 GAJ.sub.74 X.sub.75 TY.sub.75 X.sub.76 TY.sub.76 W.sub.77 GZ.sub.77 ATM.sub.78 QR.sub.79 S.sub.79 X.sub.80 TY.sub.80 X.sub.81 TY.sub.81 X.sub.82 TY.sub.82 ATM.sub.83 CAJ.sub.84 QR.sub.85 S.sub.85 TGGX.sub.87 TY.sub.87 GAJ.sub.88 CCL.sub.89 GTL.sub.90 CAJ.sub.91 TTK.sub.92 X.sub.93 TY.sub.93 W.sub.94 GZ.sub.94 QR.sub.95 S.sub.95 GTL.sub.96 TTK.sub.97 GCL.sub.98 AAK.sub.99 AAK.sub. 100 X.sub.101 TY.sub.101 GTL.sub.102 TAK.sub.103 GGL.sub.104 GCL.sub.105 QR.sub.106 S.sub.106 GAK.sub.107 QR.sub.108 S.sub.108 AAK.sub.109 GTL.sub.110 TAK.sub.111 GAK.sub.112 X.sub.113 TY.sub.113 X.sub.114 TY.sub.114 AAJ.sub.115 GAK.sub.116 X.sub.117 TY.sub.117 GAJ.sub.118 GAJ.sub.119 GGL.sub.120 ATM.sub.121 CAJ.sub.122 ACL.sub.123 X.sub.124 TY.sub.124 ATGGGL.sub.126 W.sub.127 GZ.sub.127 X.sub.128 TY.sub.128 GAJ.sub.129 GAK.sub.130 GGL.sub.131 QR.sub.132 S.sub.132 CCL.sub.133 W.sub.134 GZ.sub.134 ACL.sub.135 GGL.sub.136 CAJ.sub.137 ATM.sub.138 TTK.sub.139 AAJ.sub.140 CAJ.sub.141 ACL.sub.142 TAK.sub.143 QR.sub.144 S.sub.144 AAJ.sub.145 TTK.sub.146 GAK.sub.147 ACL.sub.148 AAK.sub.149 QR.sub.150 S.sub.150 CAK.sub.151 AAK.sub.152 CAK.sub.153 GAK.sub.154 GCL.sub.155 X.sub.156 TY.sub.156 X.sub.157 TY.sub.157 AAJ.sub.158 AAK.sub.159 TAK.sub.160 GGL.sub.161 X.sub.162 TY.sub.162 X.sub.163 TY.sub.163 TAK.sub.164 TGK.sub.165 TTK.sub.166 W.sub.167 GZ.sub.167 AAJ.sub.168 GAK.sub.169 ATGGAK.sub.171 AAJ.sub.172 GTL.sub.173 GAJ.sub.174 ACL.sub.175 TTK.sub.176 X.sub.177 TY.sub.177 W.sub.178 GZ.sub.178 ATM.sub.179 GTL.sub.180 CAJ.sub.181 TGK.sub.182 W.sub.183 GZ.sub.183 QR.sub.184 S.sub.184 GTL.sub.185 GAJ.sub.186 GGL.sub.187 QR.sub.188 S.sub.188 TGK.sub.189 GGL.sub.190 TTK.sub.191 TAGCTGCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCC-3' wherein
• A is deoxyadenyl,
• G is deoxyguanyl,
• C is deoxycytosyl,
• T is thymidyl,
• J is A or G;
• K is T or C;
• L is A,T,C or G;
• M is A, C or T;
• X is T or C, if the succeeding Y is A or G, and C if the succeeding Y is C or T;
• Y is A, G, C or T, if the preceeding X is C, and A or G if the preceding X is T;
• W is C or A, if the succeeding Z is G or A, and C if the succeeding Z is C or T;
• Z is A, G, C or T, if the preceding W is C, and A or G if the preceding W is A;
• QR is TC, if the succeeding S is A, G, C or T, and AG if the succeeding S is T or C;
• S is A, G, C or T, if the preceding QR is TC, and T or C is the preceding QR is AG and subscript numerals refer to the amino acid position in human growth hormone, for which the nucleotide sequence corresponds, according to the genetic code, the amino acid positions being numbered from the amino end.