Multilayer analytical element having an impermeable radiation nondiffusing reflecting layer
AssigneeMiles Laboratories, Inc.
InventorAlfred C. Greenquist
Life of this patent
Estimating the term…
Abstract
A multilayer analytical element for detecting a ligand in or the ligand binding capacity of a liquid sample of the type having a reagent layer(s) incorporating reagents which are responsive to the ligand in or the ligand binding capacity of the sample to give a detectable response, a radiation reflecting layer, and a support layer, the improvement wherein the reagent layer(s) is a radiation diffusing layer and the radiation reflecting layer is a radiation nondiffusing reflecting layer which is (a) interposed between the reagent layer(s) and the support layer; (b) impermeable to the ligand, reagents of the reagent layer(s), and products of their interreaction; and (c) inert to the ligand, reagents of the reagent layer(s), and products of their interreaction.
Claims29
In a multilayer analytical element for detecting a ligand in or the ligand binding capacity of a liquid sample of the type having at least one reagent layer incorporating reagents which are responsive to the ligand in or the ligand binding capacity of the sample to give a detectable response, a radiation reflecting layer, and a support layer, the improvement wherein at least one reagent layer is a radiation diffusing layer and the radiation reflecting layer is a radiation nondiffusing reflecting layer which is (a) interposed between the at least one reagent layer and the support layer;
- (b) impermeable to the ligand, reagents of the at least one reagent layer, and products of their interreaction;
- and (c) inert to the ligand, reagents of the at least one reagent layer, and products of their interreaction.
In a multilayer analytical element for detecting a ligand in or the ligand binding capacity of a liquid sample of the type having a reagent layer incorporating reagents which are responsive to the ligand in or the ligand binding capacity of the sample to give a detectable response, a radiation reflecting layer, and a support layer, the improvement wherein:
- (1) the reagent layer comprises
- (a) reagents for a homogeneous specific binding assay system which produces a detectable response that is a function of the presence of the ligand in or the ligand binding capacity of the sample;
- and
- (b) a solid carrier incorporated with the reagents;
- and
- (2) the radiation reflecting layer comprises a radiation impermeable reflective metallic layer which layer is:
- (a) interposed between the reagent layer and the support layer;
- (b) impermeable to the ligand, reagents of the reagent layer, and products of their interreaction;
- and
- (c) inert to the ligand, reagents of the reagent layer, and products of their interreaction.
In a multilayer analytical element for the detection of a ligand in or the ligand binding capacity of a liquid sample of the type having a reagent layer incorporating reagents which are responsive to the ligand in or the ligand binding capacity of the sample to give a detectable response, a radiation reflecting layer, and a support layer, the improvement wherein:
- (1) the reagent layer comprises
- (a) reagents for a homogeneous specific binding assay system which produces a detectable response that is a function of the presence of the ligand in or the ligand binding capacity of the sample;
- and
- (b) a solid carrier which comprises a plurality of zones, each of which zones is incorporated with a different reagent or reagent combination of the homogeneous specific binding assay system;
- and
- (2) the radiation reflecting layer comprises a radiation impermeable reflective metallic layer which layer is
- (a) interposed between the reagent layer and the support layer;
- (b) impermeable to the ligand, reagents of the reagent layer, and products of their interreaction;
- and
- (c) inert to the ligand, reagents of the reagent layer, and products of their interreaction.
In a multilayer analytical element for the detection of a ligand in or the ligand binding capacity of a liquid sample of the type having a reagent layer incorporating reagents which are responsive to the ligand to give a detectable response, a radiation reflecting layer, and a support layer, the improvement wherein:
- (1) the reagent layer comprises
- (a) a reagent composition including
- (i) an antibody which binds the ligand,
- (ii) a conjugate of the ligand or a binding analog thereof, and a label, and
- (iii) a detectant system which interacts with the label to produce a detectable response that is different when the label conjugate is bound by the antibody compared to when it is not so bound, whereby the detectable response is a function of the presence of the ligand in the liquid sample, and
- (b) a solid carrier incorporated with said reagent composition;
- and
- (2) the radiation reflecting layer comprises a radiation impermeable reflective metallic layer which layer is
- (a) interposed between the reagent layer and the support layer;
- (b) impermeable to the ligand, reagents of the reagent layer, and products of their interreaction;
- and
- (c) inert to the ligand, reagents of the reagent layer, and products of their interreaction.
In a multilayer analytical element for the detection of phenobarbital in a liquid sample of the type having a reagent layer incorporating regents which are responsive to phenobarbital to give a detectable response, a radiation reflecting layer, and a support layer, the improvement wherein:
- (1) the reagent layer comprises:
- (a) reagents for a homogeneous specific binding assay system which produces a detectable response that is a function of the presence of phenobarbital in or the phenobarbital binding capacity of the sample;
- and
- (b) a solid carrier incorporated with the reagents;
- and
- (2) the radiation reflecting layer comprises a radiation impermeable reflective metallic layer which layer is:
- (a) interposed between said reagent layer and said support layer;
- (b) impermeable to phenobarbital, reagents of the reagent layer, and products of their interreaction;
- and
- (c) inert to phenobarbital, reagents of the reagent layer, and products of their interreaction.
Dependencies are inferred from the claim text.