Acylation of Mucor pusillus microbial rennet enzyme
AssigneeMiles Laboratories, Inc.
InventorsDennis A. Cornelius, Clifford V. Asmus, Moshe M. Sternberg
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Abstract
A method for increasing the milk coagulating activity of microbial rennet obtained from Mucor pusillus microorganisms. The method involves acylating Mucor pusillus microbial rennet enzyme with maleic, citraconic, phthalic, cis-1,2-cyclohexanedicarboxylic, 1,2,4-benzenetricarboxylic, homophthalic, 3-nitro-phthalic, bromomaleic or dichloromaleic anhydride.
Claims2
A method for increasing the milk coagulating activity of microbial enzyme obtained from Mucor pusillus micro-organisms comprising acylating in an aqueous medium said enzyme by contacting the Mucor pusillus microbial rennet enzyme at a temperature of about 0.degree. to 25.degree. C. and a pH of about 5 10 with from 0.1 to 2 percent (w/v) based on enzyme volume of an acylating agent selected from the group consisting of maleic, citraconic, phthalic, cis-1,2-cyclohexanedicarboxylic, 1,2,4-benzenetricarboxylic, homophthalic, 3-nitrophthalic, bromomaleic and dichloromaleic anhydride for a time sufficient to obtain said desired increase.
Dependencies are inferred from the claim text.