Apparatus for high pressure peptide synthesis
AssigneeBio Research Inc.
InventorsMichael S. Verlander, William D. Fuller, Murray Goodman
Life of this patent
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Abstract
An apparatus for synthesizing a polypeptide chain on an insoluble solid support in a continuous flow reactor by performing a series of stepwise coupling reactions under high pressure is disclosed. A deprotecting agent reservoir, a solvent reservoir, a neutralizing solution reservoir, and a plurality of amino acid reservoirs are provided. Each reservoir can be selectively coupled to the reactor and a source of high pressure inert gas or an appropriate pump can be used to force the fluids as needed to the reactor. The reactor also includes a recycle loop whereby the amino acid from a particular reservoir can be recycled through the reactor under high pressure.
Claims9
An apparatus for synthesizing a polypeptide chain on an insoluble solid support by performing a series of stepwise coupling reactions under high pressure, the apparatus comprising:
- means defining a supply of deprotecting agent thereby forming a deprotecting agent reservoir;
- means defining a supply of solvent thereby forming a solvent reservoir;
- means defining a supply of neutralizing agent thereby forming a neutralizing reservoir;
- means defining a supply of at least one amino acid thereby forming at least one amino acid reservoir;
- a continuous flow high pressure reactor substantially packed with an insoluble support;
- means to selectively couple each said reservoir fluidly to said reactor;
- a high pressure pumping means for transfering the fluids contained in said reservoirs through said reactor under high pressure;
- a recycle loop means for recycling back through said reactor the amino acid which is delivered to said reactor, said recycle loop means including means defining a reagent reservoir in which the fluid being recycled is accumulated, a recycle pump means for transfering the fluid accumulated in said reagent reservoir back to said reactor, and a means for maintaining said reactor under high pressure during recycling such that the synthesis of the polypeptide chain occurs in said reactor under a pressure of at least about 40 psi.
Dependencies are inferred from the claim text.