Agglutination immunoassays carried out with agent to reduce non-specific interferences
AssigneeTechnicon Instruments Corporation
InventorsFloris de Steenwinkel, Daniel Collet-Cassart, Pierre L. Masson
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Abstract
In particle agglutination immunoassays for an analyte (e.g. an antigen or antibody) in a liquid sample (e.g. human serum), interferences arise due to non-specific protein-protein interactions and the like. These interferences are reduced or overcome by including in the assay mixture a chaotropic or chaotropic-like agent, in a carefully controlled amount. Such agents include guanidine, guanidinium hydrochloride or thiocyanate, sodium or ammonium thiocyanate, and urea, sodium chloride and ethylenediamine tetraacetic acid.
Claims19
A method of immunoassay for an analyte in a liquid sample, which comprises forming a mixture of the liquid sample with finely divided particles comprising a protein and a reagent, whereby specific agglutination or the particles occurs to an extent dependent on the amount of analyte present, and determining said extent and thereby the amount of analyte present, wherein the mixture also includes one or more agents to reduce non-specific protein interaction interferences, the amount of said agent(s) being sufficient to reduce the effect on agglutination of said interferences, wherein said agents comprise at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide.
A method of immunoassay for an analyte in a liquid sample, which comprises forming a mixture of the liquid sample with finely divided particles comprising a protein and a reagent, whereby specific agglutination of the particles occurs to an extent dependent on the amount of analyte present, and determining the said extent and thereby the amount of analyte present, wherein
- (a) as a preliminary step, solutions of the same analyte as is under assay are mixed with varying amounts of an agent comprising at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide and said particles, and the solutions assayed to determine the optimum range of said agent to reduce non-specific protein interaction interferences whilst retaining the desired immunospecific reaction upon which the assay is based;
- and then
- (b) assaying the analyte in said liquid sample in the presence of said agent in an amount within said range.
A particle agglutination specific binding assay composition for the non-specific protein interaction interference-free determination of an analyte in a liquid sample, which composition comprises:
- a particle to which is bound protein and a specific binding partner for said analyte;
- and
- an agent comprising at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide.
A particle agglutination specific binding assay method for the non-specific protein interference-free determination of an analyte in a liquid sample, which method comprises:
- contacting said liquid sample with a composition which comprises a particle to which is bound protein and a specific binding partner for said analyte and an agent which comprises at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide;
- and, thereafter, observing any particle agglutination which occurs.
Dependencies are inferred from the claim text.