Sandwich immunoassay and compositions for use therein
AssigneePalo Alto Medical Research Foundation
InventorsJack S. Remington, Fausto G. Araujo
Life of this patent
Estimating the term…
Abstract
An improved sandwich immunoassay for Toxoplasma gondii antigens associated with acute forms of Toxoplasma infection is disclosed. The assay employs either an F(ab').sub.2 fragment of IgG which reacts specifically with such antigens or a monoclonal antibody for such antigens as the substrate layer in the sandwich. The assay is sensitive and, unlike prior sandwich assays for the antigen, is not affected significantly by cross-reactions with rheumatoid factor.
Claims6
An immunoassay for detecting Toxoplasma gondii antigen associated with an acute form of Toxoplasma infection in a sample of an animal body fluid comprosing the steps of:
- (a) adsorbing an F(ab').sub.2 fragment of IgG Toxoplasma gondii antibody or a monoclonal antibody for antigens of Toxoplasma gondii onto a solid surface;
- (b) adding the sample to the adsorbed fragment of step (a) and incubating the resulting mixture;
- (c) separating the adsorbed fraction from the non-adsorbed fraction of the incubated mixture of step (b);
- (d) adding a labeled Toxoplasma gondii antibody to the adsorbed fraction of step (c) and incubating the resulting mixture;
- (e) separating the adsorbed fraction from the non-adsorbed fraction of the incubated mixture of step (d);
- and
- (f) determining the presence of labeled Toxoplasma gondii antibody in the adsorbed fraction of step (e).
Dependencies are inferred from the claim text.