Ultrapurification of factor VIII using monoclonal antibodies
AssigneeScripps Clinic and Research Foundation
InventorsTheodore S. Zimmerman, Carol A. Fulcher
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Abstract
A method of preparing high purity procoagulant protein comprising the steps of (a) adsorbing a VIII:C/VIII:RP complex from a plasma or commercial concentrate source of factor VIII onto agarose beads bound to a monoclonal antibody specific to VIII:RP, (b) eluting VIII:C with a salt solution, (c) adsorbing the eluted VIII:C on an animohexyl agarose column and eluting the VIII:C with a salt solution.
Claims16
An improved method of preparing Factor VIII procoagulant activity protein comprising the steps of
- (a) adsorbing a VIII:C/VIII:RP complex from a plasma or commercial concentrate source onto particles bound to a monoclonal antibody specific to VIII:RP,
- (b) eluting the VIII:C,
- (c) adsorbing the VIII:C obtained in step (b) in another adsorption to concentrate and further purify same,
- (d) eluting the adsorbed VIII:C, and
- (e) recovering highly purified and concentrated VIII:C.
An improved immunoadsorbent for isolation and purification of VIII:C from VIII:C/VIII:RP comprising a monoclonal antibody specific to VIII:RP bound to solid particles.
In a method for purifying Factor VIII procoagulant activity protein from plasma or concentrate, the improvement comprising the step of passing said plasma or concentrate through a chromatographic type column having adsorbent to which is bound monoclonal antibodies which is specific to VIII:RP and eluting the VIII-C therefrom.
Dependencies are inferred from the claim text.