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US 4,360,597 AGranted
Streptomyces plasmid and culture
AssigneeNational Research Development Corporation
InventorsMervyn J. Bibb, David A. Hopwood
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AssigneeNational Research Development Corporation
InventorsMervyn J. Bibb, David A. Hopwood
Estimating the term…
Novel Streptomyces plasmids have the characteristic that their presence in non-integrated form in a micro-organism of the species Streptomyces lividans confers on that micro-organism the properties (a) of forming "pocks" when grown on a "lawn" of that strain of micro-organism deposited with the National Collection of Industrial Bacteria (NCIB) under the reference number 11416, and (b) of not forming "pocks" when grown on a "lawn" of that strain of micro-organism deposited with the NCIB under the reference number 11417, or which is derivable from a plasmid having such a characteristic by the removal or addition of DNA therefrom. The plasmids are prepared from micro-organisms containing them or by the manipulation of other plasmids of the group and are of value as vectors for the introduction of nucleic acid into micro-organisms.
A Streptomyces plasmid which has the characteristic that it comprises that portion of the DNA sequence of the plasmid, designated SLP 1.6 and shown in the Figure, required for the expression of replication and that its presence in non-integrated form in a microorganism of the species Streptomyces lividans confers on that microorganism the properties (a) of forming "pocks" when grown on a "lawn" of that strain of microorganism deposited with the NCIB under the reference number 11416, and (b) of not forming "pocks" when grown on a "lawn" of that strain of microorganism deposited with the NCIB under the reference number 11417, said plasmid being in isolated form.
A Streptomyces plasmid selected from the group consisting of the plasmids isolable, respectively, from the strains of microorganism deposited with the NCIB under the reference numbers 11417, 11499, 11500, 11501, 11502, and 11503, said plasmid having the structural features given in the following Table
A Streptomyces plasmid which comprises that portion of the DNA sequence of the plasmid, designated SLP 1.6 and shown in the Figure, required for the expression of replication and lethal zygosis, said plasmid being in isolated form.
A biologically pure culture of a microorganism of the species Streptomyces lividans characterized by the properties (a) of forming "pocks" when grown on a "lawn" of that strain of microorganism deposited with the NCIB under the reference member 11416, and (b) of not forming "pocks" when grown on a "lawn" of that strain of microorganism deposited with the NCIB under the reference number 11417.
A biologically pure culture of a strain of Streptomyces lividans selected from the group consisting of those strains deposited with the NCIB under the reference numbers 11417, 11499, 11500, 11501, 11502 and 11503.
Dependencies are inferred from the claim text.