Process of producing a peptide antibiotic with Bacillus circulans
AssigneeBristol-Myers Company
InventorsMasataka Konishi, Takeo Miyaki, Hiroshi Tsukiura, Hiroshi Kawaguchi
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Abstract
A novel peptide antibiotic complex designated herein as Bu-2470 is produced by fermentation of Bacillus circulans strain G493-B6 (ATCC 31,805). Complex Bu-2470 may be separated into four bioactive peptide antibiotics designated as Bu-2470A, B.sub.1, B.sub.2a, and B.sub.2b. The complex and individual bioactive factors possess significant antimicrobial activity.
Claims9
A process for producing the peptide antibiotic Bu-2470A of the formula ##STR7## wherein Dab represents .alpha.,.gamma.-diaminobutyric acid, Leu represents leucine and Phe represents phenylaline which comprises cultivating a Bu-2470A-producing strain of Bacillus circulans under submerged aerobic conditions in an aqueous nutrient medium containing assimilable sources of nitrogen and carbon until a substantial amount of Bu-2470A is produced by said organism in said culture medium and then recovering the Bu-2470A antibiotic from the culture medium substantially free of co-produced substances.
A process for producing the peptide antibiotic Bu-2470B.sub.1 of the formula ##STR8## Dab represents .alpha.,.gamma.-diaminobutyric acid, Leu represents leucine and Phe represents phenylalanine which comprises cultivating a Bu-2470B.sub.1 -producing strain of Bacillus circulans under submerged aerobic conditions in an aqueous nutrient medium containing assimilable sources of nitrogen and carbon until a substantial amount of Bu-2470B.sub.1 is produced by said organism in said culture medium and then recovering the Bu-2470B.sub.1 antibiotic from the culture medium substantially free of co-produced substances.
A process for producing the peptide antibiotic Bu-2470B.sub.2a of the formula ##STR9## Dab represents .alpha.,.gamma.-diaminobutyric acid, Leu represents leucine and Phe represents phenylalanine comprises cultivating a Bu-2470B.sub.2a -producing strain of Bacillus circulans under submerged aerobic conditions in an aqueous nutrient medium containing assimilable sources of nitrogen and carbon until a substantial amount of Bu-2470B.sub.2a is produced by said organism in said culture medium and then recovering the Bu-2470B.sub.2a antibiotic from the culture medium substantially free of co-produced substances.
A process for producing the peptide antibiotic Bu-2470B.sub.2b of the formula ##STR10## Dab represents .alpha.,.gamma.-diaminobutyric acid, Leu represents leucine and Phe represents phenylalanine which comprises cultivating a Bu-2470B.sub.2b -producing strain of Bacillus circulans under submerged aerobic conditions in an aqueous nutrient medium containing assimilable sources of nitrogen and carbon until a substantial amount of Bu-2470B.sub.2b is produced by said organism in said culture medium and then recovering the Bu-2470B.sub.2b antibiotic from the culture medium substantially free of co-produced substances.
Dependencies are inferred from the claim text.