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US 4,360,592 AGranted
Process for the detection of antibodies
OwnerJoel K. Weltmaninventor
Life of this patent
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Abstract
Novel enzyme conjugates useful in immunoassay methods are prepared with the use of a novel coupling reagent of N-succinimidyl (4-iodoacetyl) aminobenzoate by reacting the coupling reagent, firstly, with an amino-containing macromolecule, and, thereafter, with a sulfhydryl-containing enzyme, the enzyme conjugate prepared in a high yield and of high specificity.
Claims15
In a process for the detection and determination of a macromolecular substance in a biological fluid, which process comprises:
- (a) providing a solid support material which adheres specifically to the macromolecular substance to be detected and determined:
- (b) contacting the support material with a biological fluid containing the macromolecular substance, to bind the macromolecular substance to the support material;
- (c) removing the unbound substance of the biological fluid;
- (d) adding an enzyme conjugate to the support material containing the bound macromolecular substance, to bind the enzyme conjugate in proportion to the amount of the bound macromolecular substance;
- and
- (e) determining the amount of the bound macromolecular substance by the activity of the bound enzyme conjugate, the improvement which comprises:
- employing, as the enzyme conjugate, an enzyme-conjugate compound having an enzymatically active enzyme and an immuno-active macromolecule enzymatically linked by a coupling reagent and having the formula:
- ##STR11## wherein n is a whole number of from 1 to 8.
5 more elements
In a process for the detection and determination of an immunoglobulin macromolecular substance in a biological fluid, which process comprises:
- (a) providing a solid support material which adheres specifically to the macromolecular substance to be detected and determined;
- (b) contacting the support material with a biological fluid containing the macromolecular substance, to bind the macromolecular substance to the support material;
- (c) removing the unbound substance of the biological fluid;
- (d) adding a beta-D-galactosidase enzyme conjugate to the support material containing the bound macromolecular substance, to bind the enzyme conjugate in proportion to the amount of the bound macromolecular substance;
- and
- (e) determining the amount of the bound macromolecular substance by the activity of the bound enzyme conjugate, the improvement which comprises:
- employing, as the enzyme conjugate, an enzyme-conjugate compound having an enzymatically active enzyme and an immuno-active macromolecule enzymatically linked by a coupling reagent and having the formula:
- ##STR15##
5 more elements
Dependencies are inferred from the claim text.