Process for the determination of free fatty acids

In a process for determining the presence of free fatty acids in a system comprising a first reaction (1) in which an acyl coenzyme A synthetase is reacted with said free fatty acids in the presence of adenosine triphosphate and a coenzyme A to yield an acyl coenzyme A and a second reaction (2) in which said acyl coenzyme A produced in the first reaction is reacted with an acyl coenzyme A oxidase to form hydrogen peroxide and determining the amount of free fatty acids by correlating the amount of hydrogen peroxide produced to the amount of fatty acids present in the system, the improvement in which myokinase is added to the first reaction system in an amount ranging from about 1 to about 20 units, said amount being sufficient to promote the rapid and complete formation of the acyl coenzyme A in the first reaction, which in turn permits a rapid and precise determination of the amount of free fatty acids in the system.