Autonomously replicating DNA containing inserted DNA sequences
OwnerGeorge Pieczenikinventor
Life of this patent
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Abstract
Autonomously replicating DNA containing a unique nucleotide sequence, being an oligonucleotide of which its sequence does not otherwise exist in said DNA, inserted in a non-essential region thereof at a site not previously susceptible of restriction endonuclease cleavage.
Claims13
A population of linear DNA molecules of an autonomously replicating DNA element, randomly cleaved, comprising circular permutations of the same nucleotide sequence, to the ends of which have been covalently attached an oligonucleotide of unique sequence, being an oligonucleotide of which its sequence does not otherwise exist in said DNA element.
An autonomously replicating, transferable DNA comprising a unique nucleotide sequence, being an oligonucleotide of which its sequence does not otherwise exist in said transferable DNA, inserted in a non-essential region thereof at a site not previously susceptible of restriction endonuclease cleavage.
An autonomously replicating, transferable DNA comprising a unique nucleotide sequence, being an oligonucleotide of which its sequence does not otherwise exist in said transferrable DNA inserted in a structural gene thereof at a site not previously susceptible of restriction endonuclease cleavage.
A sequencing vector comprising an autonomously replicating DNA element having an insert comprising a unique restriction site, being a restriction site of which its sequence does not otherwise exist in the DNA element, adjacent to a unique nucleotide sequence of which its sequence does not otherwise exist in the DNA element, said unique restriction site being located on the 5' side of said unique nucleotide sequence.
Dependencies are inferred from the claim text.