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US 4,359,527 AGranted
Cancer diagnostic assay
AssigneeChildren's Hospital Medical Center
InventorBruce R. Zetter
Life of this patent
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Abstract
In vitro cancer diagnostic assay comprising providing a substratum coated with a layer of visible particles susceptible to ingestion by capillary endothelial cells, plating such cells onto the substratum, allowing the cells to adhere, incubating the cells with a test sample, measuring the area of the visible particle-depleted phagokinetic track left by at least one of the cells, and comparing that area to the track area left by a control cell, a comparatively larger test track area indicating the presence in the test sample of a factor associated with cancer cells.
Claims6
An in vitro cancer diagnostic assay comprising
- providing a substratum coated with a layer of visible particles susceptible to ingestion by capillary endothelial cells,
- plating capillary endothelial cells onto said substratum,
- allowing said capillary endothelial cells to adhere to said substratum,
- incubating said adhered cells with medium comprising a test sample to be assayed for the presence of a factor associated with cancer cells,
- measuring the area of the visible particle-depleted phagokinetic track left by at least one of said capillary endothelial cells, and
- comparing said area with the phagokinetic track area left by at least one control capillary endothelial cell incubated under the same conditions, but in the absence of said test sample,
- a comparatively larger phagokinetic track area left by said cells incubated with said test sample indicating the presence in said test sample of said factor associated with cancer cells.
4 more elements
Dependencies are inferred from the claim text.