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US 4,359,389 AGranted
Method for the purification of interferon
AssigneeStichting Rega VZW
InventorJochen W. Heine
Life of this patent
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Abstract
Human fibroblast interferon may be purified to a high degree by using a simple two-step purification method comprising (a) subjecting an aqueous interferon solution to chromatography on porous glass beads, and (b) subjecting the resulting aqueous interferon solution to chromatography on immobilized zinc chelate. Overall recoveries of about 45-76% of the initial interferon activity may be achieved and the end product will be free of any skin reactive agents.
Claims14
A method for the purification of interferon, by chromatography comprising the steps of:
- (a) contacting an aqueous solution of human fibroblast interferon with porous glass beads at neutral or slightly alkaline pH for selective adsorption of interferon from solution onto the beads;
- (b) contacting the beads with an elution agent at acidic pH for elution of adsorbed interferon from said beads;
- (c) collecting a first interferon-containing eluate;
- (d) contacting said eluate with immobilized zinc chelate at neutral or slightly alkaline pH for selective adsorption of interferon from said eluate onto said zinc chelate;
- (e) contacting said zinc chelate with an elution agent at acidic pH for elution of interferon from said chelate;
- and
- (f) recovering a second eluate containing purified interferon.
4 more elements
Dependencies are inferred from the claim text.