4-Chloro-7-sulphobenzofurazan and salts thereof; new fluorigenic thiol specific reagents

Abstract

New fluorigenic thiol specific reagents, 4-chloro-7-sulphobenzofurazan and salts thereof, are disclosed. The reagents are prepared by sulphonation of 4-chlorobenzofurazan. Ammonium 4-chloro-7-sulphobenzofurazan is used to label glutathione as a model thiol peptide and bovine serum albumin and jackbean urease as thiol containing proteins.

Dependencies are inferred from the claim text.