Throw-away subculturing device
AssigneeBecton, Dickinson and Company
InventorGilbert S. Bittings
Life of this patent
Estimating the term…
Abstract
A device is provided for blind subculturing in place of a standard petri dish, which device eliminates the need for a separate needle and syringe. The device includes a culture media in combination with an absorbent fiber disc adjacent the culture media for absorbing inoculum and maintaining it adjacent the media gel. In addition, a mounted needle is included in the device for penetrating the septum of a standard culture bottle, for withdrawing the inoculum. Means are provided for readily removing the culture media from the device momentarily for inspection, with the means including a cooperating closure assembly on the device utilizing a conventional petri plate as the top and/or bottom cap or closure.
Claims9
A throw-away subculture device, characterized by
- (a) a container defining an enclosure with a top opening and a bottom opening;
- (b) a top cap for said top opening;
- (c) a bottom cap for said bottom opening;
- (d) a web spaced from said top cap dividing said enclosure into two chambers one of which defines a subculture area in said enclosure;
- (e) at least one well and culture media in said top cap for cooperation with said subculture area;
- (f) at least one absorbent fiber disc mounted adjacent said well for receiving inoculum introduced into said subculture area;
- and
- (g) at least one needle mounted in said web and extending therethrough for introducing inoculum to said subculture area, the point of said needle extending toward said bottom cap.
A throw-away subculture device characterized by:
- (a) a tubular container open at the top and the bottom, said container comprised of a semi-rigid material;
- (b) a top cap for said container;
- (c) a bottom cap for said container;
- (d) cooperating angular surfaces between said container and said top and bottom caps;
- (e) a well in said top cap defining a media container for said device;
- (f) a web integral with said tubular container walls and mounted spaced from said top opening dividing said container into two chambers one of which defines a subculture area in said container;
- (g) an absorbent fiber disc in said subculture area for receiving inoculum introduced into said subculture area;
- and
- (h) a needle supported in said web and extending therethrough, with the point thereof extending toward said bottom opening.
Dependencies are inferred from the claim text.