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US 4,358,535 AGranted
Specific DNA probes in diagnostic microbiology
AssigneeThe Board of Regents of the University of Washington
InventorsStanley Falkow, Stephen L. Moseley
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AssigneeThe Board of Regents of the University of Washington
InventorsStanley Falkow, Stephen L. Moseley
Estimating the term…
Method and compositions for infectious disease diagnosis and epidemiology involving labeled nucleotide probes complementary to nucleic acid coding for a characteristic pathogen product. Clinical isolates are cultivated, expanding the number of microorganisms, the resulting colonies lysed, the genome normally denatured and then fixed. Alternatively, clinical samples (stool, sputum, pus, etc.) are spotted onto an inert support. The sample is treated in such a way that the DNA is liberated from microbes present in the sample and complexed onto the support. The DNA is normally denatured and fixed in this process. Subsequently, a labelled polynucleotide probe specific for a DNA sequence characteristic of a pathogenic product suspected of being present in the clinical sample is contacted with the fixed genomic single stranded nucleic acid under hybridizing conditions. Hybridization of probes to the single stranded nucleic acid is diagnostic of the presence of the pathogen.
A method for detecting the presence of a pathogen in a clinical sample suspected of containing said pathogen, said method comprising:
A method for detecting the presence of a unicellular pathogen in a sample suspected of containing said pathogen, said method comprising:
A method for detecting the presence of a gram negative bacillus in a clinical isolate suspected of containing said bacillus, said method comprising:
Dependencies are inferred from the claim text.