Phosphopeptides from casein-based material

A method for recovering phosphopeptides from a casein-based material containing phosphocaseinates of monovalent cations or paracasein derived therefrom comprising subjecting said casein-based material to an enzymatic hydrolysis at a PH in the range of 7 to 9 and a temperature in the range of 37.degree. to 45.degree. C. by means of at least one proteolytic enzyme capable of substantially reproducing the proteic digestion which occurs in vivo in the human body, subjecting the resultant hydrolyzate to at least one ultrafiltration step on membranes which retain the enzyme but allow all the peptides of the hydrolyzate to pass in the permeate to obtain a permeate containing phosphopeptides and non-phosphorylated peptides, adding to the permeate at least one bivalent cation salt to form aggregates of the phosphopeptides to produce a solution containing essentially the aggregates of phosphopeptides and the non-phosphorylated peptides, subjecting the solution to at least one ultrafiltration step with membranes that retain the aggregates of phosphopeptides and allow the non-phosphorylated peptides to pass through, and recovering the retained phosphopeptides.