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US 4,357,421 AGranted
Synthetic gene coding for influenza hemagglutinin
AssigneeG. D. Searle & Co.
InventorsJohn S. Emtage, Norman H. Carey
Life of this patent
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Abstract
A process for the production of a synthetic gene for an influenza haemagglutinin which comprises: (a) subjecting isolated vRNA to the action of reverse transcriptase to produce a double-stranded RNA/DNA hybrid; (b) digesting the RNA component of the hybrid; (c) treating the residual DNA component of the hybrid to produce a double-stranded DNA molecule having one "hairpin" end; and (d) treating the "hairpin" structure with a single-strand specific nuclease to produce a bimolecular double-stranded copy of the vRNA.
Claims20
A process for the production of a synthetic gene for an influenza haemagglutinin comprising:
- (a) subjecting isolated influenza vRNA to the action of reverse transcriptase to produce a double-stranded RNA/DNA hybrid;
- (b) digesting the RNA component of the hybrid;
- (c) treating the residual DNA component of the hybrid to produce a double-stranded DNA molecule having one "hairpin" end;
- and
- (d) treating the "hairpin" structure with a single strand specific nuclease to produce a bimolecular double-stranded copy of the vRNA.
2 more elements
Dependencies are inferred from the claim text.