Polysaccharide RBS substance and antitumor agent containing same
AssigneesSapporo Breweries Limited, Etsuo Ito, Daicel Chemical Industries, Ltd.
InventorsEichi Soma, Kohei Kobayashi, Takuro Karakawa, Shigeyoshi Kato, Kiichi Uchida
Life of this patent
Estimating the term…
Abstract
A polysaccharide RBS substance having the ultraviolet absorption spectrum depicted in FIG. 1 and the infrared absorption spectrum depicited in FIG. 2 is obtained from rice bran by extraction with hot water. These substances have been tested for anti-tumor activity against Sarcoma 180 ascites tumor and against Ehrlich ascites tumor and were determined to have anti-tumor activity against these tested tumors.
Claims2
A polysaccharide RBS substance and pharmaceutically acceptable addition salts thereof (1) having the following properties:
- it does not pass through a semipermeable membrane;
- it is insoluble in alcohol, acetone, hexane, benzene, ethyl acetate, dimethylsulfoxide, ligroin, carbon tetrachloride, chloroform and ether, and is soluble or highly soluble in water;
- a 1% aqueous solution or suspension is neutral or slightly acidic;
- it reacts positively in the following reactions:
- the Molisch reaction, the anthrone-sulfuric acid reaction, the tryptophane-sulfuric acid reaction, the crystein-sulfuric acid reaction, the chromotrope-sulfuric acid reaction, the phenol-sulfuric acid reaction, the carbazole-sulfuric acid reaction, the biuret reaction, the ninhydrin reaction and the Lowry-Folin reaction;
- and negatively in the Elson-Morgan reaction and the starch-iodine reaction;
- it has the ultraviolet absorption spectrum depicted in FIG. 1 and the infrared absorption spectrum depicted in FIG. 2;
- and
- (2) containing glucose units as the sugar component, said glucose units being bonded by .alpha.-1,6 glucoside bonds as the main chain and said main chain is branched.
A pharmaceutical composition effective in inhibiting the growth of Sarcoma 180 ascites tumors, Erlich ascites tumors, and tumor cells (SV 40-transformed C3H-2K cells) consisting essentially of polysaccharide RBS substance and pharmaceutically acceptable acid-addition salts thereof in a pharmaceutically effective amount, and a pharmaceutically acceptable vehicle, said polysaccharide RBS substance (1) having the following properties:
- it does not pass through a semipermeable membrane;
- it is insoluble in alcohol, acetone, hexane, benzene, ethyl acetate, dimethylsulfoxide, ligroin, carbon tetrachloride, chloroform and ether, and is soluble or highly soluble in water;
- a 1% aqueous solution or suspension is neutral or slightly acidic;
- it reacts positively in the following reactions:
- the Molisch reaction, the anthrone-sulfuric acid reaction, the tryptophane-sulfuric acid reaction, the crystein-sulfuric acid reaction, the chromotrope-sulfuric acid reaction, the phenol-sulfuric acid reaction, the carbazole-sulfuric acid reaction, the biuret reaction, the ninhydrin reaction and the Lowry-Folin reaction;
- and negatively in the Elson-Morgan reaction and the starch-iodine reaction;
- it has the ultraviolet absorption spectrum depicted in FIG. 1 and the infrared absorption spectrum depicted in FIG. 2;
- and
- (2) containing glucose units as the sugar component, said glucose units being bonded by .alpha.-1,6-glucoside bonds as the main chain and said main chain is branched.
Dependencies are inferred from the claim text.