Method and composition for reducing the nonspecific binding of radioiodinated protein hormones
AssigneeBaxter Travenol Laboratories, Inc.
InventorsCatherine T. Chan, Debra A. Gravallese
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Abstract
Nonspecific binding of radioiodinated protein hormones in specific-receptor binding assays is reduced by including a nonionic detergent with the tracer during storage prior to the assay.
Claims19
In a method for the immunoassay of a hormone selected from the group consisting of prolactin, insulin, gonadotropin, growth hormone, ACTH, thyrotropin and parahormone, wherein said hormone in radioiodinated form is used as a tracer, the improvement comprising a stabilized tracer reagent consisting essentially of said radioiodinated hormone and about from 0.05 to 0.5% w/v of a nonionic detergent.
A composition consisting essentially of a radioiodinated hormone from the group consisting of prolactin, insulin, gonadotropin, growth hormone, ACTH, thyrotropin and parahormone, and about from 0.05 to 0.5% w/v of a nonionic detergent, said composition being essentially free of hormone receptor and unlabelled hormone.
Dependencies are inferred from the claim text.