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US 4,352,885 AGranted
Preparation of a novel NADP linked alcohol-aldehyde/ketone oxidoreductase from thermophilic anaerobic bacteria for analytical and commercial use
AssigneeWisconsin Alumni Research Foundation
InventorsJoseph G. Zeikus, Raphael J. Lamed
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Abstract
A partially purified NADP specific thermostable alcohol, aldehyde/ketone oxidoreductase is prepared which can react with a wide range of alcohols, ketones and aldehydes. The enzyme has a unique preference for secondary over primary alcohols. The thermostability, broad range of operating temperatures and lack of sensitivity to metal ions and complexing agents, in addition to the absolute specifity for the coenzyme, increase the utility of the enzyme in asymmetric organic synthesis and NADPH regeneration.
Claims6
A method for producing thermostable enzymes, said method comprising the steps of:
- (a) inoculating a culture medium with a strain of thermophilic bacteria selected from the group consisting of Thermoanaerobium brockii and Clostridium thermohydrosulfuricum;
- (b) incubating said bacteria at a temperature between 50.degree. C. and 75.degree. C.;
- (c) purifying the bacteria from spent culture supernatant fluids by heating to a temperature in the range of 85.degree.-98.degree. C.;
- and
- (d) isolating from the bacteria thermostable NADP linked alcohol, aldehyde/ketone oxidoreductases that are formed.
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Dependencies are inferred from the claim text.