Carrier having acrylate copolymer coating for immobilization of bioactive materials
AssigneeKuraray Co., Ltd.
InventorsToshihide Nakashima, Masao Tanihara, Koichi Takakura
Life of this patent
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Abstract
Carriers coated with a copolymer of a hydrophilic acrylate or methacrylate monomer and a copolymerizable unsaturated carboxylic acid or unsaturated amine are provided which are substantially free from nonspecific adsorption of proteins and the like. Due to the side-chain carboxyl or amino groups of the copolymer, these coated carriers are able to immobilize bio-active materials such as antigens, antibodies, complements and enzymes. These carriers, on which bio-active materials can be immobilized, are useful as clinical selective adsorbents, affinity-chromatographic adsorbents, selective electrodes or columns for analysis.
Claims14
A carrier for immobilization of bio-active materials which comprises a base material coated with a copolymer of (i) a hydrophilic acrylate or methacrylate monomer of the general formula CH.sub.2 =(R.sub.1)CO.sub.2 R.sub.2 OR.sub.3, wherein R.sub.1 is H or methyl;
- R.sub.2 is a substituted or unsubstituted divalent alkylene radical of 2 to 3 carbon atoms or a poly(oxyalkylene) radical;
- R.sub.3 is H or an alkyl radical of 1 to 3 carbon atoms, which alkyl radical can be further substituted by polar substituent groups and (ii) a copolymerizable unsaturated carboxylic acid of the general formula CH.sub.2 =C(R.sub.1)CO.sub.2 H, wherein R.sub.1 is H or methyl or (iii) a copolymerizable unsaturated amine of the general formula CH.sub.2 =C(R.sub.1)CO.sub.2 R.sub.2 NHR.sub.3, wherein R.sub.1 is H or methyl;
- R.sub.2 is a divalent alkylene radical of 2 or 3 carbon atoms;
- and R.sub.3 is H or an alkyl radical of 1 to 3 carbon atoms, said copolymerizable component being present in amount of from about 1 to 50 weight percent based on the total weight of monomers.
Dependencies are inferred from the claim text.