Aminopyrine improved Trinder's reagent and dosing process for hydrogen peroxide from enzymatic oxidation of metabolic substrata with the same
AssigneeE.L.V.I. S.p.A.
InventorsUgo De Luca, Francesco Zoppi
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Abstract
An aminopyrine-improved Trinder's reagent is disclosed to be used for the dosing of hydrogen peroxide from enzymatic oxidation of metabolic substrata, such as glucose, cholesterol, uric acid, tryglycerides and coline. The use of such an improved reagent in Trinder's reaction enables to avoid any interference from bilirubin, particularly in the determination of uric acid. The dosing process (for uric acid) is also disclosed by means of such a reagent.
Claims3
In a Trinder's reagent for the clinical analysis of hydrogen peroxide by the enzymatic oxidation of a metabolic substrate in the presence of bilirubin, said reagent containing 2,4-dichlorophenyl sulphonate and 4-aminophenazone;
- the improvement in which the Trinder's reagent contains 1 to 30 g/l of aminopyrine to block the interferring action of said bilirubin.
Dependencies are inferred from the claim text.