Tumor localization and therapy with labeled anti-CEA antibody
OwnerMilton D. Goldenberginventor
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Abstract
Improved methods are provided for using radiolabeled antibodies to carcinoembronic antigen (CEA) to locate, diagnose and stage CEA-containing tumors by external photoscanning, whereby significantly increased resolution, convenience and/or efficiency of operation may be achieved. A method is provided for using highly specific radiolabeled anti-CEA antibodies for tumor therapy. An injectable composition and radiolabeled antibodies are provided for use in the method of the invention.
Claims39
In a method for determining the location of a tumor which either produces or is associated with carcinoembryonic antigen (CEA), which comprises injecting a human subject parenterally with an antibody specific to CEA and radiolabeled with a pharmacologically inert radioisotope capable of detection using a photoscanning device and subsequently scanning the subject with said device to determine the location of the resultant uptake of said labeled antibody by said tumor;
- the improvement which comprises concurrently injecting said human subject with normal immunoglobulin from the same or different species as that used to prepare said specific antibody, said normal immunoglobulin being radiolabeled with a different radioisotope of the same element used to label the specific antibody and emitting at an energy capable of independent detection using said photoscanning device, the level of activity of the labeled normal immunoglobulin being used to determine the distribution of background activity due to non-targeted specific antibody, said distribution being subtracted from the total activity of specific antibody whereby the activity of substantially only the targeted tumor-associated antibody is determined.
In a method for determining the location of a tumor which either produces or is associated with carcinoembryonic antigen, which comprises injecting a human subject parenterally with an antibody specific to CEA and radiolabeled with a pharmacologically inert radioisotope capable of detection using a photoscanning device and subsequently scanning the subject with said device to determine the location of the resultant uptake of said labeled antibody by said tumor;
- the improvement which comprises using as said anti-CEA antibody a substantially monospecific antibody having a CEA-specific immunoreactivity prior to labeling of at least 70% and a cross-reactivity to other antigens of less than 15%, said antibody being radiolabeled to an extent sufficient to reduce its CEA-specific immunoreactivity by from 5 to 33%.
Substantially monospecific anti-CEA antibody having a CEA-specific immunoreactivity prior to labeling of at least 70% and a cross-reactivity to other antigens of less than 15%, said antibody being radiolabeled with a pharmacologically inert radioisotope capable of detection using a photoscanning device, the extent of radiolabeling being sufficient to reduce said CEA-specific immunoreactivity by from 5 to 33%.
An injectable composition which comprises
- (a) an antibody specific to carcinoembryonic antigen and radiolabeled with a pharmaceutically inert radioisotope capable of detection using a photoscanning device;
- (b) normal immunoglobulin from the same or different species as that used to prepare said specific antibody, said normal immunoglobulin being radiolabeled with a different isotope of the same element used to label the specific antibody and emitting at an energy capable of independent detection using said photoscanning device;
- and
- (c) a pharmaceutically acceptable injection vehicle.
An injectable composition which comprises
- (a) substantially monospecific anti-CEA antibody having a CEA-specific immunoreactivity prior to labeling of at least 70% and a cross-reactivity to other antigens of less than 15%, said antibody being radiolabeled with a pharmacologically inert radioisotope capable of detection using a photoscanning device, the extent of radiolabeling being sufficient to reduce said CEA-specific immunoreactivity by from 5 to 33%;
- and
- (b) a pharmaceutically acceptable injection vehicle.
A method of tumor radiotherapy, which comprises parenterally injecting into a human subject having a tumor which produces or is associated with carcinoembryonic antigen (CEA) a tumor-reducing amount of an antibody which is specific to CEA and radiolabeled with a pharmacologically inert, radiotherapeutically effective radioisotope;
- wherein said antibody is substantially monospecific to CEA, having a CEA-specific immunoreactivity prior to labeling of at least 70% and a cross-reactivity to other antigens of less than 15%.
A method of tumor radiotherapy, which comprises the steps of parenterally injecting into a human subject having a tumor which produces or is associated with CEA a radiotherapeutically effective amount of an antibody which is specific to CEA and radiolabeled with a pharmacologically inert radioisotope capable of detection with a photoscanning device, said labeled antibody further containing in chemical combination an addend containing at least five atoms of boron with at least a natural abundance of Boron-10 isotope;
- locating said tumor by scanning the subject with said photoscanning device to determine the location of the resultant uptake of said labeled antibody by said tumor;
- and directing a beam of thermal neutrons at said tumor location.
An antibody which is specific to carcinoembryonic antigen, said antibody being radiolabeled with a pharmacologically inert radioisotope capable of detection with a photoscanning device, said labeled antibody further containing in chemical combination an addend containing at least five atoms of boron with at least a natural abundance of Boron-10 isotope.
Dependencies are inferred from the claim text.