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AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsToru Nakanishi, Yozo Machida
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The substrate for xanthine oxidase such as xanthine, hypoxanthine, guanine, 2-hydroxypurine, etc. are determined by oxidizing the substrate by the action of xanthine oxidase derived from a microorganism and determining the formed hydrogen peroxide by the system for the determination of hydrogen peroxide comprising peroxidase and coloring reagent. The determination is carried out in one step by using the test composition for the determination of the substrate for xanthine oxidase which comprises xanthine oxidase and a system for the determination of hydrogen peroxide. The determination method can be applied to the determination of the compound which participates in the system wherein the substrate is stoichiometrically formed by the reaction or decomposition. When the system is an enzymatic reaction, the compound is determined in one step. Xanthine oxidase used in the present invention is different from known xanthine oxidase in molecular weight and is prepared by culturing a microorganism belonging to the genus Enterobacter.
Xanthine oxidase having the following enzymological properties:
A process for producing xanthine oxidase which comprises culturing a microorganism belonging to the genus Enterobacter and capable of producing xanthine oxidase in a nutrient medium, forming xanthine oxidase in the culture liquor and recovering the same therefrom.
Dependencies are inferred from the claim text.