Preparation of coupled hemoglobin molecules
AssigneeBiotest-Serum-Institut GmbH
InventorsKlaus Bonhard, Uwe Boysen
Life of this patent
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Abstract
Hemoglobin molecules are coupled so as to increase their intravascular residence times without significantly diminishing their oxygen transport ability. This is achieved by coupling hemoglobin molecules to one another and/or to serum proteins and gelatin derivatives using dialdehydes such as aliphatic dialdehydes of 3 to 8 carbon atoms, optionally followed by addition of pyridoxal phosphate. The desired material isolated by ammonium sulfate precipitation, the sulfate being added before, simultaneously with, or after the dialdehyde. Solution of the precipitate followed by dialysis and/or ion exchange can also be undertaken.
Claims12
A chemically modified, stable hemoglobin preparation suitable for blood replacement and for organ perfusion, comprising hemoglobin molecules coupled to one another or to another protein selected from the group consisting of serum proteins and gelatin derivatives by a coupling reagent comprising an aliphatic dialdehyde having 3 to 8 carbon atoms, molar ratio of the coupling reagent to the hemoglobin being about 6:1 to about 18:1, the preparation having a capacity to transport oxygen corresponding approximately to that of free hemoglobin, and a blood residence time which is at least about twice that of free hemoglobin.
Dependencies are inferred from the claim text.