Method of growing anchorage-dependent cells
AssigneeMonsanto Company
InventorsWilliam R. Tolbert, Mary M. Hitt, Joseph Feder, Richard C. Kimes
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Abstract
Anchorage-dependent cells are grown in agitated microcarrier suspension in which the cells and microcarriers are aggregated by periodically providing a temporary residence of said microcarriers and cells outside the main cell culture reactor agitation zone and in a separate compartment wherein they are subjected to a gentle tumbling action within a confined space having substantially the same environmental conditions as in the main cell culture reactor.
Claims10
In a method of growing anchorage-dependent cells in agitated microcarrier suspension culture, the improvement comprising aggregating the microcarriers and cells by periodically providing a temporary residence of said microcarriers and cells outside the main cell culture reactor agitation zone and in a separate compartment wherein they are subjected to a gentle tumbling action within a confined space having substantially the same environmental conditions as in said main cell culture reactor agitation zone and thereafter recycling said microcarriers and cells to said main cell culture reactor agitation zone.
Dependencies are inferred from the claim text.