Process for purifying particles of biological origin, particularly the surface antigen of the virus of hepatitis B (AgHBs)
AssigneeInstitut Pasteur
InventorsPhilippe J. Adamowicz, Alberte Platel nee Bonnet, Ludwig Muller
Life of this patent
Estimating the term…
Abstract
The invention relates to a process for purifying biological particles, particularly for purifying the surface antigen (HBs) of the hepatitis B virus. A suspension of this HBs antigen is subjected to rate zonal banding in a gradient inside a centrifuge equipped with a continuous-flow core. At the same time as the centrifugation operation is carried out, the suspension of particles to be purified is caused to circulate in a closed circuit in which the centrifuge is inserted. The band containing the purified biological particles is then collected in a way known per se.
Claims16
In a process for purifying or concentrating biological particles in a liquid composition containing said biological particles in a solution or suspension wherein said liquid composition is subjected to rate zonal banding by centrifugation in a gradient within a centrifuge, the improvement which comprises purifying said liquid composition by continuously circulating said liquid composition in a closed circuit into and out from the centrifuge, wherein said centrifuge is part of said closed circuit, the continuous circulation is carried out at the same time as the centrifugation is carried out and the rate and period of circulation are such that said liquid composition is recirculated through said centrifuge and part of the particles in said liquid composition is collected by the gradient at each passage.
Dependencies are inferred from the claim text.