Loading patent…
AssigneeUnitika Ltd.
InventorsHiroshi Nakajima, Kazuhiko Nagata, Masao Kageyama, Toyohiko Suga, Tadao Suzuki, Kenzo Motosugi
Estimating the term…
Strain UK 788 (FERM-P No. 5141) that belongs to Bacillus stearothermophilus, the cell of which is longer than about 10 microns and which permits easier release of intracellular components than a type culture Bacillus stearothermophilus, IAM 11001 is disclosed. Also, a process for producing a useful enzyme selected from the group consisting of a heat-resistant polynucleotide phosphorylase, heat-resistant maleate dehydrogenase, heat-resistant glucokinase, heat-resistant glucose-6-phosphate dehydrogenase and heat-resistant pyruvate kinase by culturing such UK 788 and recovering the desired enzyme from the culture is disclosed. Since the cell of the strain UK 788 is easy to settle and its membrane is also easy to break, the useful enzymes mentioned above can be efficiently produced on an industrial scale.
A biologically pure culture of strain FERM-P No. 5141 of Bacillus stearothermophilus, the cell of which is longer than about 10 microns and which permits easier release of an intracellular component than a standard strain Bacillus stearothermophilus IAM 11001.
A process of extracting an intracellular component from strain FERM-P No. 5141, comprising culturing cells of strain FERM-P No. 5141 of Bacillus stearothermophilus and recovering the desired intracellular component from the culture.
A process for producing a useful enzyme selected from the group consisting of a heat-resistant polynucleotide phosphorylase, heat-resistant maleate dehydrogenase, heat-resistant glucokinase, heat-resistant glucose-6-phosphate dehydrogenase and heat-resistant pyruvate kinase comprising culturing cells of strain FERM-P No. 5141 of Bacillus stearothermophilus and recovering the desired enzyme from the culture.
Dependencies are inferred from the claim text.