Stabilization of peroxidase
AssigneeAkzona Incorporated
InventorsEdward C. Dawson, Jan D. H. Homan, Bauke K. Van Weemen
Life of this patent
Estimating the term…
Abstract
New and useful stable peroxidase compositions are disclosed which are particularly useful as reagents in enzyme immunoassay (EIA) tests. The novel peroxidase compositions contain polyvalent ions of groups 3 and 4 of the Periodic Table. These novel compositions are stable even in low peroxidase concentrations, and are relatively invulnerable to freeze-drying. Preferably, the polyvalent ions selected are those of Al, Zn, Mg, Fe, and Cu.
Claims12
A method of manufacturing a composition useful as a diagnostic tool for enzyme immunoassay, comprising:
- (a) freezing at a temperature from about -40 Deg. F. to about -50 Deg. F. an aqueous peroxidase composition containing (1) peroxidase in an amount from about 1 nanogram per milliliter to about 25 micrograms per milliliter, and (2) at least one polyvalent metal salt selected from the group consisting of the sulphate, the phosphate, the halide, and nitrate of any of Mg, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Ar, Zn, Ga, and Al present in a peroxidase-stabilizing amount of from about 0.0001 M to about 0.05 M to form a frozen ice;
- and
- (b) subliming said frozen ice under reduced pressure to form a freeze-dried composition.
A method of manufacturing a composition useful as a diagnostic tool for enzyme immunoassay, comprising:
- (a) freezing at a temperature from about -40 Deg. F. to about -50 Deg. F. an aqueous peroxidase composition consisting essentially of (1) peroxidase in an amount from about 1 nanogram per milliliter to about 25 micrograms per milliliter, and (2) at least one polyvalent metal salt selected from the group consisting of the sulphate, the phosphate, the halide, and nitrate of any of Mg, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Ar, Zn, Ga, and Al present in a peroxidase-stabilizing amount of from about 0.0001 M to about 0.05 M to form a frozen ice;
- and
- (b) subliming said frozen ice under reduced pressure to form a freeze-dried composition.
Dependencies are inferred from the claim text.