Method of producing a plaque dispersing enzyme
AssigneeThe United States of America as represented by the Secretary of the Navy
InventorsLloyd G. Simonson, Burton L. Lamberts
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Abstract
A method of producing .alpha.-1, 3-glucanase by introducing a bacterial culture such as Pseudomonas sp. isolate NRRL B-12324 into an aqueous medium containing a "limit glucan" substrate which is greater than 90 percent .alpha.-1, 3-glycosidically linked, then allowing growth to take place to accumulate .alpha.-1, 3-glucanase, and then recovering the enzyme for use as an oral therapeutic agent.
Claims5
A method for producing a plaque-dispersing .alpha.-1, 3-glucan 3-glucanohydrolase (1,3-glucanase) enzyme which comprises cultivating a bacterial culture of the strain Pseudomonas NRRL B-12324 in an aqueous medium containing a "limit glucan" substrate which is greater than 90 percent .alpha.-1, 3-glycosidically linked, then allowing growth until the medium contains an accumulation of 1,3-glucanase and then recovering said enzyme.
A method for producing a plaque-dispersing 1,3-glucanase enzyme which comprises cultivating a strain Pseudomonas NRRL B-12324 in an aqueous medium containing a "limit glucan" substrate which is greater than 90 percent .alpha.-1, 3-glycosidically linked at a temperature of about 30 degrees C. for a period of at least one day and then recovering said enzyme.
Dependencies are inferred from the claim text.