Process for the production of pyruvic acid and citric acid
AssigneeDiamond Shamrock Corporation
InventorRefaat M. El-Sayed
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Abstract
Process for the production of citric acid, characterized in that E. Coli KG 93, F.sup.- is cultivated in a first step for 15-24 hours at a temperature of 20.degree.-37.degree. C. and a pH of 5.0-7.5 on a substrate consisting of whey permeate to which has been added phosphates in a content of 0.8-1.6 g/l and nitrates in a content of 0.8-1.2 g/l or a corresponding quantity of urea, that H. Wickerhamii CBS 4308 in a second step is cultivated for 20-26 hours at a temperature of 15.degree.-35.degree. C. and a pH of 4.5-6.5 on the cultivating solution from the first step, whereupon citric acid is obtained from the cultivating solution in a way known per se.
Claims74
Process for the production of citric acid, characterized in that E. Coli KG 93, F.sup.- is cultivated in a first step for 15-24 hours at a temperature of 20.degree.-37.degree. C. and a pH of 5.0-7.5 on a substrate consisting of whey permeate to which has been added phosphates in a content of 0.8-1.6 g/l and nitrates in a content of 0.8-1.2 g/l or a corresponding quantity of urea, that H. Wickerhamii CBS 4308 in a second step is cultivated for 20-26 hours at a temperature of 15.degree.-35.degree. C. and a pH of 4.5-6.5 on the cultivating solution from the first step, whereupon citric acid is obtained from the cultivating solution in a way known per se.
A process for producing pyruvic acid which comprising cultivating in a medium containing a source of lactose carbon a strain of Escherichia Coli which is capable of producing pyruvic acid from lactose carbon and has substantially the same identifying characteristics as Escherichia Coli DSM 1392 until pyruvic acid is accumulated in a recoverable amount, said cultivation being under aerobic conditions at the beginning and later under anerobic conditions.
A process for the production of citric acid which comprises cultivating on a pyruvic acid-containing cultivation medium a strain of Hansenula Wickerhamii which is capable of forming citric acid from pyruvic acid, the cultivation medium being achieved by cultivating a strain of Escherichia Coli being capable of forming pyruvic acid on a lactose-containing medium, the cultivation of H. Wickerhamii being effected under conditions inhibiting metabolism of pyruvic acid to carbon dioxide, and thereafter recovering citric acid from the resulting cultivation broth.
A process for the production of citric acid which comprises cultivating a strain of a microorganism having the identifying characteristics of Escherichia Coli DSM 1392 in an aqueous culture medium containing lactose as a carbon source until pyruvic acid is accumulated under conditions which include aerobic cultivation for at least a first part of the civilization, and cultivating in the resulting medium containing pyruvic acid a strain of microorganism having the identifying characteristics of Hansenula Wickerhamii DSM 1380 until citric acid is accumulated in said resulting medium.
The process of claim 65 wherein the said first medium contains whey permeate, from 0.8 to 1.6 grams/liter of phosphate ion, from 0.8 to 1.2 grams/liter of nitrate ion and the pH of the said first medium is 5 to 7.5.
In a process for producing citric acid which comprises cultivating a microorganism in a medium containing pyruvic acid, the step of producing the pyruvic acid which comprises cultivating a strain of a microorganism having the identifying characteristics of Escherichia Coli DSM 1392 in a culture medium containing lactose carbon until pyruvic acid is accumulated.
In a process for producing citric acid which comprising cultivating a microorganism in a nutrient culture medium containing pyruvic acid, the step of cultivating a strain of a microorganism having the identifying characteristics of Hansenula Wickerhamii DSM 1380 in said culture medium until citric acid is accumulated.
A biologically pure culture of Escherichia Coli DSM 1392 in an aqueous medium containing a nutrient which is convertible into pyruvic acid by the Escherichia Coli DSM 1392.
A biologically pure aqueous culture medium containing lactose, a microorganism having the identifying characteristics of Escherichia Coli DSM 1392 and pyruvic acid.
A biologically pure aqueous culture medium containing pyruvic acid and a microorganism having the identifying characteristics of Hansenula Wickerhamii DSM 1380.
A biologically pure aqueous culture medium containing lactose, Escherichia Coli DSM 1392, pyruvic acid and Hansenula Wickerhamii DSM 1380.
A biologically pure culture of the microorganism Escherichia Coli DSM 1392.
A biologically pure culture of the microorganism Escherichia Coli DSM 1392, said culture being capable of accumulating pyruvic acid in a recoverable quantity upon fermentation in an aqueous nutrient medium containing lactose.
A biologically pure culture of the microorganism Hansenula Wickerhamii DSM 1380.
A biologically pure culture of the microorganism Hansenula Wickerhamii DSM 1380, said culture being capable of accumulating citric acid in a recoverable quantity upon fermentation in an aqueous nutrient medium containing pyruvic acid.
Dependencies are inferred from the claim text.