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US 4,321,397 AGranted
4-Aminoantipyrine dye for the analytic determination of hydrogen peroxide
AssigneeMillipore Corporation
InventorsPaul T. Nix, Spencer M. Fields
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Abstract
A 3-aminobenzoic acid or a 3-aminobenzenesulfonic acid, when reacted with a 4-aminoantipyrine in aqueous medium in the presence of hydrogen peroxide forms a novel colored dye which is believed to be a charge-transfer complex of free radicals formed from the 3-aminoaromatic acid and the 4-aminoantipyrine. The dye and the process by which it is produced are useful in analytical procedures for hydrogen peroxide, as well as in enzymatic analyses involving the formation of hydrogen peroxide.
Claims5
A dye compound produced by a method which comprises:
- contacting in an aqueous medium at a temperature of from about 20.degree. to about 45.degree. C., having a pH of from about 3 to about 9.5, and containing hydrogen peroxide,
- (1) peroxidase,
- (2) an aminoaromatic acid represented by the formula:
- ##STR7## wherein X is a mono or dialkylamino group having 1 to 6 carbons in each alkyl and ZH is the carboxyl or the sulfo group, and
- (3) a 4-aminoantipyrine represented by the formula:
- ##STR8## wherein each of R and R.sup.1, when taken separately, is alkyl of 1 to 6 carbons, R.sup.2 is phenyl or substituted phenyl wherein the substituents are lower alkyl or halogen, each of R.sup.3 and R.sup.4, when taken separately is hydrogen or alkyl of 1 to 6 carbons, and Y is oxygen or sulfur.
3 more elements
Dependencies are inferred from the claim text.