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US 4,320,196 AGranted
Semi-synthesis of human insulin
AssigneeShionogi & Co., Ltd.
InventorsKazuyuki Morihara, Tatsushi Oka, Hiroshige Tsuzuki
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Abstract
A process for preparing a B30-threonine-insulin which comprising reacting a des-B30-insulin with an excess amount of threonine derivative in the presence of an enzyme specifically acting on the basic-amino-acid carbonyl in peptide bondings.
Claims14
A process for preparing a B30-threonine-insulin which comprises reacting a des-B30-insulin with a threonine derivative of the formula
- Thr(R.sup.1)(R.sup.2)
- wherein Thr is an L-threonine residue, R.sup.1 is hydrogen or a hydroxy-protecting group and R.sup.2 is a carboxyl-protecting group, in a molar ratio of the threonine derivative to the des-B30-insulin of 5:1 to 1000:1, and in the presence of an enzyme specifically acting on the basic-amino-acid carbonyl in peptide bondings selected from the group consisting of trypsin, a trypsin-like enzyme produced by Streptomyces fradiae ATCC 3535 and a trypsin-like enzyme produced by Streptomyces erythreus, and subjecting the resultant product to one or more reactions to remove all of the protecting groups.
Dependencies are inferred from the claim text.