Process for producing cyclodextrins
AssigneeSanraku-Ocean Co., Ltd.
InventorsYoshiaki Yagi, Kageaki Kouno, Taiji Inui
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Abstract
A process for producing cyclodextrins which comprises using cultured medium of the strains belonging to genus Micrococcus, filtrate thereof or enzyme preparation obtained therefrom, especially, the process in which the ratio of .alpha.-cyclodextrin to .beta.-cyclodextrin can be desirably varied by regulating the condition of the reaction mixture, is disclosed.
Claims8
A process for producing cyclodextrins which comprises reacting starch or degraded starch as a substrate with a cyclodextrin glycosyltransferase enzyme preparation selected from the group consisting of cultured medium, cultured medium filtrate, concentrate thereof or enzyme preparation which is obtained by cultivating microorganisms belonging to genus Micrococcus capable of producing cyclodextrin glycosyltransferase, to provide cyclodextrins at a pH and a temperature effective for said reaction.
A process for producing cyclodextrin glycosyltransferase which comprises cultivating aerobically a microorganism belonging to genus Micrococcus capable of producing cyclodextrin glycosyltransferase in a nutrient medium under the condition of pH 7-10.5, at the temperature of 25.degree.-40.degree. C. for 24-96 hours, and effecting purification of the product of the cultivation step by the use of a procedure selected from the group consisting of concentration of the cultured medium, salting out, fractional precipitation, adsorption, elution, precipitation, isoelectric precipitation, electric dialysis independently and a combination thereof.
Dependencies are inferred from the claim text.