Process for the production of fructose transferase enzyme

An improved process for producing a fructosyl transferase enzyme preparation which comprises:

• inoculating a culture medium with cells from a strain of Aureobasidium pullulans, said culture medium comprising about 16 to about 24% (w/v) sucrose, about 1 to about 2.4% (w/v) yeast extract and about 1% (w/v) of an inorganic nitrate salt;
• and culturing the mixture at a pH of between 6 and 8 at 28.degree.-32.degree. C. until a yield of fructosyl transferase enzyme is obtained, wherein the enzyme preparation is recovered in high yields from the culture medium without the need to remove any black pigment or viscous polysacchride.