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US 4,309,418 AGranted
Anti-tumor agent from human serum and process
AssigneeSloan-Kettering Research Institute for Cancer
InventorSaul Green
Life of this patent
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Abstract
The invention relates to an anti-tumor agent prepared from human serum by the process steps of multiple salting out, centrifuging, desalting and gel filtration.
Claims14
Process for preparing an anti-tumor agent from buffered human serum which comprises:
- (i) removing a first portion of proteins from the serum by addition of ammonium sulfate salt in an amount up to about 35% of the amount necessary to form a saturated solution while maintaining the pH at 7;
- thereafter
- (ii) centrifuging the serum at a force of 105,000 g to separate the first portion of proteins, and discarding the first portion of proteins;
- (iii) recovering a second portion of proteins from the serum by addition of ammonium sulfate salt in an amount to total an amount greater than about 35% up to about 70% of the amount necessary to form a saturated solution while maintaining the pH at 7, and retaining the second portion of proteins;
- (iv) desalting the second portion of proteins;
- (v) gel filtering the desalted proteins;
- and
- (vi) collecting a gel filtered active fraction having a molecular weight greater than 150,000.
5 more elements
Anti-tumor agent derived from human serum comprising a protein fraction having a molecular weight of more than 150,000 which is stable at 90.degree. C. for at least one hour, non-dialyzable and inhibits the growth of HeLa tumor cells.
Dependencies are inferred from the claim text.