Leucine-rich 3,1-S-.alpha..sub.2 -glycoprotein, process for isolating it and its use
AssigneeBehringwerke Aktiengesellschaft
InventorsHeinz Haupt, Siegfried Baudner
Life of this patent
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Abstract
New glycoprotein having the following properties: a sedimentation constant in the ultracentrifuge of 3.1 S.+-.0.2; molecular weight 49,600.+-.4,000; electrophoretic mobility in the range of the .alpha..sub.2 -globulins of the human serum; isoelectric range pH 3.8 to 4.1; extinction coefficient E 1 cm, 280 nm=1% 7.0.+-.0.2; content of about 77.+-.2% of .alpha.-amino acids with a proportion of 17.+-.2% of leucine and about 22% of carbohydrates; specific immunological reaction with antibodies directed against the glycoprotein; which can be isolated from human sera and which may be used for the preparation of antisera enabling the detection and determination of the glycoprotein in body fluids.
Claims5
Glycoprotein isolated from human serum, characterized by the following parameters:
- (a) sedimentation constant in the ultracentrifuge 3.1 S.+-.0.2;
- (b) molecular weight of 49,000.+-.4,000;
- (c) electrophoretic mobility in the range of the .alpha..sub.2 -globulins of the human serum;
- (d) isoelectric range pH 3.8-4.1;
- (e) extinction coefficient E.sub.1 cm.sup.1%, 280 nm=7.0.+-.0.2;
- (f) content of about 77%.+-.2% of .alpha.-amino-acids and about 23%.+-.2% of carbohydrates, and of said amino acids and carbohydrates, the major fractions being present as follows:
- ______________________________________ Mole % of the amino-acid pro- Variation Amino Acids portion coefficient % ______________________________________ Aspartic acid 11.45 1.49 Serine 6.43 2.36 Glutamic acid 10.72 2.51 Proline 7.04 4.43 Glycine 7.32 2.31 Alanine 6.65 1.97 Leucine 22.05 5.79 ______________________________________ Carbohydrate Residues % by weight ______________________________________ Galactose 4.1 .+-. 0.3 Mannose 3.8 .+-. 0.3 N-Acetylhexosamine 8.1 .+-. 0.5 N-Acetylneuraminic acid 6.6 .+-. 0.4 ______________________________________
- (g) specific immunological reactions with antibodies directed against the glycoprotein.
Glycoprotein isolated from human serum, characterized by the following parameters:
- (a) sedimentation constant in the ultracentrifuge 3.1 S.+-.0.2;
- (b) molecular weight of 49,000.+-.4,000;
- (c) electrophoretic mobility in the range of the .alpha..sub.2 -globulins of the human serum;
- (d) isoelectric range pH 3.8-4.1;
- (e) extinction coefficient E.sub.1 cm.sup.1%, 280 nm=7.0.+-.0.2;
- (f) content of about 77%.+-.2% of .alpha.-amino-acids and about 23%.+-.2% of carbohydrates, said amino acids and carbohydrates being present as follows:
- ______________________________________ Mole % of the amino-acid pro- Variation Amino Acids portion coefficient % ______________________________________ Lysine 3.87 5.90 Hystidine 2.23 11.28 Arginine 4.65 7.68 Aspartic acid 11.45 1.49 Threonine 3.87 6.26 Serine 6.43 2.36 Glutamic acid 10.72 2.51 Proline 7.04 4.43 Glycine 7.32 2.31 Alanine 6.65 1.97 1/2 Cystine 1.21 9.83 Valine 4.48 3.78 Methionine 0.75 5.81 Isoleucine 1.29 3.16 Leucine 22.05 5.79 Tyrosine 0.81 19.53 Phenylalanine 3.23 1.39 Tryptophane 1.60 11.69 ______________________________________ Carbohydrate Residues % by weight ______________________________________ Galactose 4.1 .+-. 0.3 Mannose 3.8 .+-. 0.3 Glucose 0 Fucose 0.3 .+-. 0.1 Xylose 0 N-Acetylhexosamine 8.1 .+-. 0.5 N-Acetylneuraminic acid 6.6 .+-. 0.4 ______________________________________
- (g) specific immunological reactions with antibodies directed against the glycoprotein.
Dependencies are inferred from the claim text.