Method for producing cacao butter substitute

In a method for producing fats and oils rich in 1,3-disaturated-2-unsaturated-triglycerides useful as a cacao butter substitute, comprising cultivating a microorganism being capable of assimilating a higher alkyl derivative and producing fats and oils containing 1,3-disaturated-2-unsaturated-triglycerides in a medium under an aerobic condition, collecting the cells of the microorganism and recovering the fats and oils from the cells, the improvement wherein the medium contains a mixed carbon source comprising at least one stearyl compound selected from the group consisting of an alkyl stearate having 1 to 4 carbon atoms in the alkyl moiety, vinyl stearate, stearyl acetate, stearyl alcohol, stearyl chloride and ammonium stearate, and at least one other carbon compound selected from the group consisting of a palmityl compound, an oleyl compound and a saccharide.