Isomerization of glucose to fructose using glucose isomerase from ampullariella
AssigneeThe Dow Chemical Company
InventorsSharonkay E. Foley, Patrick J. Oriel, Carol C. Epstein
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Abstract
The production of a heat stable glucose isomerase from a microorganism belonging to the genus Ampullariella and a method for using the isomerase to convert glucose to fructose.
Claims27
A process for producing glucose isomerase from a microorganism belonging to the genus Ampullariella which comprises growing the Ampullariella in a culture medium and recovering the glucose isomerase therefrom.
A process for isomerizing D-glucose to D-fructose including the step of treating the D-glucose in an aqueous medium with an isomerase produced by a microorganism belonging to the genus Ampullariella thereby converting a portion of the glucose to fructose.
A biologically pure culture of the microorganism Ampullariella species 3876 demonstrating glucose isomerase activity having the identifying characteristics of ATCC 31351, said culture producing glucose isomerase in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and essential minerals.
A biologically pure culture of the microorganism Ampullariella species 3877 demonstrating glucose isomerase activity having the identifying characteristics of ATCC 31352, said culture producing glucose isomerase in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and essential minerals.
A biologically pure culture of the microorganism Ampullariella species 3965 demonstrating glucose isomerase activity having the identifying characteristics of ATCC 31353, said culture producing glucose isomerase in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and essential minerals.
A biologically pure culture of the microorganism Ampullariella species 3966 demonstrating glucose isomerase activity having the identifying characteristics of ATCC 31354, said culture producing glucose isomerase in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and essential minerals.
A culture medium consisting essentially of a biologically pure microorganism selected from the group consisting of Ampullariella species 3876, Ampullariella species 3877, Ampullariella species 3965 and Ampullariella species 3966 or a mutant thereof and an aqueous nutrient culture medium containing assimilable sources of carbon, nitrogen, and essential minerals, said culture medium wherein glucose isomerase is produced in a recoverable quantity upon aerobic fermentation.
Dependencies are inferred from the claim text.