Test for bacteria
AssigneeLaboratoire de Recherche API
InventorDaniel Monget
Life of this patent
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Abstract
A test for the detection of bacteria of the genuses Salmonella and Serratia and distinguishing them from the genuses Proteus and Providencia carried out in the presence of a diazonium salt and a synthetic enzymatic substrate in the form of an ester having an aliphatic chain of 7 to 10 carbon atoms. Two reactions may be effected in the same reactive medium. The test may be associated with other tests such as the .beta.-glucosidase, .beta.-galactosidase and .beta.-glucuronidase research tests making it possible to apply it simultaneously to the detection of bacteria belonging to other genuses: Kliebsiella, Enterobacter, Escherichia.
Claims5
A method for the detection of bacteria of the genuses Salmonella and Serratia and for distinguishing said bacteria from bacteria of the genuses Proteus and Providencia which comprises the steps of:
- (a) wetting a substrate with an aqueous medium having a pH close to neutral wherein said substrate comprises a C.sub.7 to C.sub.10 aliphatic ester of a compound selected from the group consisting of 1-naphthol, 2-naphthol, o-nitrophenol, p-nitrophenol, a hydroxy-coumarin, an indoxyl, a 4-methyl-umbelliferole, fluorescein, phenolphthaleine, esculetin, and a hydroxy-quinoline;
- (b) contacting the substrate with a cultured colony of bacteria suspected to belong to the genuses Salmonella and Serratia;
- (c) incubating the colony of bacteria;
- (d) contacting the colony of bacteria and the substrate with a diazonium salt capable of a color change indicating hydrolysis of the C.sub.7 to C.sub.10 aliphatic ester defined in step (a);
- and
- (e) relating any change in color to the presence of either the genuses Salmonella and Serratia or to the genuses Proteus and Providencia.
Dependencies are inferred from the claim text.