Material able to fix reversibly biologial macromolecules, its preparation and its application

A process for the preparation of a porous solid material for use in a chromatography column and capable of fixing reversibly biological macromolecules comprising:

• (1) coating a porous inorganic support with a polysaccharide polymer having .alpha.-glycol groups which undergo oxidative scission, said polysaccharide polymer being selected from the group consisting of polysaccharide polymer, per se and a modified polysaccharide polymer of the formula ##STR4## wherein R represents the polysaccharide polymer residue, n is a whole number ranging from 1 to 10 and R.sub.1 and R.sub.2 each independently represent lower alkyl or hydroxy lower alkyl;
• (2) subjecting said polysaccharide polymer to an oxidative scission reaction to produce a polymer having the formula R.sub.4 --CHO wherein R.sub.4 is the residue of the resulting oxidized polysaccharide polymer;
• (3) reacting said resulting polymer with an amino molecule or macromolecule having the formula R'--NH.sub.2 wherein R' is the residue of said amino molecule or macromolecule, to produce a polysaccharide polymer having the formula R.sub.4 --CH.dbd.N--R' wherein R.sub.4 and R' have the meanings given above.
• (4) and reducing the imine bond in the polysaccharide polymer resulting from step (3) to a stable amine bond.