Anticoagulant stopper coating
AssigneeSherwood Medical Industries Inc.
InventorsRonald Crouther, Fred E. Satchell, Glen Stone
Life of this patent
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Abstract
A stopper for a blood collection tube adapted to receive whole blood for centrifugal separation into its relatively light and heavy phases. The stopper is provided with a coating which prevents red blood cells from adhering to it so as to avoid red cell contamination of the lighter phase after phase separation. The stopper is coated with a cationic binding agent, such as tridodecylmethylammonium chloride, and then with an anionic anticoagulant such as dextran sulfate. A lubricant, such as silicone oil, is applied to the coated stopper to facilitate insertion of the stopper into the tube.
Claims33
A stopper for a blood collection container capable of sealingly closing one end of the container with a bottom surface thereof facing the opposed end of the container comprising an elastomeric closure member, a first layer of a binding agent covering said bottom surface, and a second layer including dextran sulfate bonded to and covering said first layer to inhibit adherence of red blood cells to said closure member when contacted by blood.
In a blood collection device including a blood collection tube, and a stopper closing one end of the tube, the improvement comprising a first layer of a cationic surfactant binding agent on at least the bottom surface of the stopper within the tube, a second layer including dextran sulfate covering said first layer and being bound to said first layer such that it is substantially insoluble in whole blood, said second layer inhibiting adherence of red blood cells to the stopper during use of the device.
The method of treating an elastomeric closure stopper for a blood collection container to inhibit the adhesion of red blood cells thereto during use comprising the steps of coating at least that portion of the stopper that is subject to come in contact with blood with a solution of a binding agent, drying the stopper to effect a dry binding agent layer, coating the binding agent layer with a solution including dextran sulfate, and drying the stopper to effect a dried layer including dextran sulfate bonded to the binding agent layer, to inhibit red blood cell adhesion to the stopper when contacted by blood.
Dependencies are inferred from the claim text.