Method and composition for direct determination of iron in blood serum
AssigneeR.C.C. Societa' Ricerche di Chimica Clinica S.r.l.
InventorFerruccio A. R. Ceriotti
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Abstract
A direct method for determination of iron in blood serum comprising reacting the blood serum with ferrozine in the presence of a reducing agent to bring iron ion into divalent form, thiosemicarbazide and hydrochloric acid at a pH between 1.7 and 2.1, without any addition of buffers and tensioactive agents and determining colorimetrically the iron content of the specimen by means of the colored complex formed between iron ion and ferrozine against a reagent blank.
Claims14
A direct method for determination of iron in blood serum comprising (a) reacting the blood serum with ferrozine in the presence of a reducing agent to bring iron ion into divalent form, thiosemicarbazide and hydrochloric acid at a pH between 1.7 and 2.1, without any addition of buffers and tensioactive agents and (b) determining colorimetrically the iron content of the specimen by means of the colored complex formed between iron ion and ferrozine against a reagent blank.
In a method for direct colorimetric determination of iron in blood serum by reacting the serum iron with ferrozine in the presence of acid and a reducing agent to release serum iron from transferrin and reduce iron ion to divalent form the improvement which comprises:
- carrying out the iron-ferrozine reaction in the presence of sufficient thiosemicarbazide to eliminate significant copper interference, without any addition of buffer and tensioaction agents and at a pH sufficiently low that such elimination of copper interference is obtained without significant reaction of the thiosemicarbazide with iron, said low pH also being such that substantially complete formation of a colored iron-ferrozine complex is obtained.
A kit for colorimetric determination of iron in blood serum without protein precipitation characterized in that the reagent mixture for colorimetric determination comprises hydrochloric acid, ascorbic acid, ferrozine and thiosemicarbazide and does not require addition of tensioactive agents, the amounts of hydrochloric acid, ferrozine and thiosemicarbazide being such that substantially complete reaction of ferrozine with iron in serum, and substantially complete elimination of copper interference without substantial reaction of iron with thiosemicarbazide is obtained.
A reagent composition for determination of iron in blood serum consisting essentially of ferrozine, thiosemicarbazide, ascorbic acid and sufficient hydrochloric acid to provide a pH of from about 1.7 to about 2.1.
Dependencies are inferred from the claim text.