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AssigneeBayer Aktiengesellschaft
InventorsWerner Frommer, Lutz Muller, Delf Schmidt, Walter Puls, Hans-Peter Krause, Ulrich Heber
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According to the present invention inhibitors for glycoside hydrolases, and in particular glucosidase inhibitors especially saccharase inhibitors, which are active in the digestive tract are formed by culturing organisms of the family Bacillaceae, particularly by strains of the genus Bacillus. It has also been found that certain strains of organisms of the family Bacillaceae, especially strains DSM 7, DSM 704 and DSM 675 produce the antibiotic known as 1-desoxynojirimycin. The invention therefore provides a method of producing 1-desoxynojirimycin which comprises culturing a 1-desoxynojirimycin producing organism of the genus Bacillus. Inhibitors for glycoside hydrolases and the invention includes methods for producing such inhibitors, pharmaceutical compositions containing the inhibitors and methods of treatment involving the use of the inhibitors.
A method of producing an inhibitor for glycoside hydrolases which comprises culturing an organism of the family Bacillaceae capable of producing said inhibitor in a nutrient solution at about 15.degree.-40.degree. C. for non-thermophilic Bacilli and 15.degree.-80.degree. C. for thermophilic Bacilli containing a source of nitrogen, a source of carbon and a nutrient salt for a time sufficient to produce said inhibitor then separating said glycoside hydrolase inhibitor from the culture, wherein said inhibitor is:
A method of producing 1-desoxynojirimycin which comprises culturing a 1-desoxynojirimycin producing organism of the genus Bacillus in a nutrient solution at about 15.degree.-40.degree. C. for non-thermophilic Bacilli and 15.degree.-80.degree. C. for thermophilic Bacilli containing a source of nitro, a source of carbon and a nutrient salt for a time sufficient to produce said 1-desoxynojirimycin, then separating 1-desoxynojirimycin from the culture.
An in vitro biologically pure culture of a glycoside-hydrolase-inhibiting producing micro-organism of the genus Bacillus, strain DSM 704, DSM 740, DSM 741 or DSM 742.
Dependencies are inferred from the claim text.